2023

Conformational variability of cyanobacterial ChlI, the AAA+ motor of magnesium chelatase involved in chlorophyll biosynthesis

Dmitry Shvarev, Alischa Ira Scholz, Arne Moeller Magnesium chelatase is a conserved enzyme complex responsible for the first committed step of chlorophyll biosynthesis in photosynthetic organisms, which is the addition of magnesium to the chlorophyll precursor, protoporphyrin IX. The complex is composed of the catalytic subunit ChlH, the bridging subunit ChlD, and the subunit ChlI, …

Conformational variability of cyanobacterial ChlI, the AAA+ motor of magnesium chelatase involved in chlorophyll biosynthesis Read More »

Yck3 casein kinase-mediated phosphorylation determines Ivy1 localization and function at endosomes and vacuole

Sophie Grziwa, Jan-Hannes Schäfer, Raffaele Nicastro, Annabel Arens, Claudio De Virgilio, Florian Fröhlich, Arne Moeller, Jieqiong Gao, Lars Langemeyer, Christian Ungermann The casein kinase Yck3 is a central regulator at the vacuole that phosphorylates several proteins involved in membrane trafficking. Here, we set out to identify novel substrates. We localized endogenously tagged Yck3 not only …

Yck3 casein kinase-mediated phosphorylation determines Ivy1 localization and function at endosomes and vacuole Read More »

Structure of the metazoan Rab7 GEF complex Mon1–Ccz1–Bulli

Eric Herrmann, Jan-Hannes Schäfer, Stephan Wilmes, Christian Ungermann, Arne Moeller, and Daniel Kümmel The endosomal system of eukaryotic cells represents a central sorting and recycling compartment linked to metabolic signaling and the regulation of cell growth. Tightly controlled activation of Rab GTPases is required to establish the different domains of endosomes and lysosomes. In metazoans, …

Structure of the metazoan Rab7 GEF complex Mon1–Ccz1–Bulli Read More »

Regulatory sites in the Mon1-Ccz1 complex control Rab5 to Rab7 transition and endosome maturation

Ann-Christin Borchers, Maren Janz, Jan-Hannes Schäfer, Arne Moeller, Daniel Kümmel, Achim Paululat, Christian Ungermann, Lars Langemeyer Maturation from early to late endosomes depends on the exchange of their marker proteins Rab5 to Rab7. This requires Rab7 activation by its specific guanine nucleotide exchange factor (GEF) Mon1- Ccz1. Efficient GEF activity of this complex on membranes …

Regulatory sites in the Mon1-Ccz1 complex control Rab5 to Rab7 transition and endosome maturation Read More »